Made of 240ml plastic. I washed it with detergent and sterilized it with Milton after each use. Being plastic, there are fine scratches, print fading due to rubbing, and signs of use. Please check the images and consider before purchasing. I used it until last year, so it hasn't been sitting unused for long, so please rest assured. The nipple is not included for hygiene reasons. Please purchase separately. No pets, no smokers.